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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Stable DNMT3L overexpression in SH-SY5Y neurons recreates a facet of the genome-wide Down syndrome DNA methylation signature

Fig. 1

Stable overexpression of human DNMT3L in SH-SY5Y cells at different phases of neural differentiation. a PiggyBac vector for stable integration of human DNMT3L (hDNMT3L), which was co-expressed with eGFP. Upon translation the self-cleaving peptide (P2A) sequence between the polypeptide resulted in separate proteins. The vector was flanked by inverted terminal repeats (ITRs) that were recognized by the PiggyBac transposon. b Representative live cell images the DNMT3L + eGFP and eGFP cell lines during the different phases assayed in either the GFP or phase contrast channels. c Western blot of DNMT3L and GAPDH in wild-type SH-SY5Y cells (WT) and the transgenic cell lines

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