Fig. 4

Rbm10 is important for heterochromatin silencing. a Serial dilutions of indicated cells harboring ura4⁺ at the otr region were plated in the PMG minimal medium without uracil (-Ura) or the EMM medium with the counter-selective FOA. Ctrl, a control plate-containing uracil. WT, wild type. b Serial dilutions of rbm10Δ cells and WT as control carrying his3⁺ at the telomeric region (tel) were plated in the PMG medium without histidine (-His). c Growth assays of rbm10Δ and WT cells carrying ura4⁺ at the mating-type region in the PMG medium without uracil (-Ura) or with the FOA media. d Analysis of H3K9 methylation in the otr region by ChIP. ChIP assays were performed using an antibody against H3K9 di-methylation and primers specific for in the otr region. act1⁺ was used as a control