Fig. 2

Overexpression of Rbm10 results in severe growth defects and massive intron retention. a Cells overexpressing Rbm10 display slow growth. Serial dilutions of indicated cells carrying pREP1-rbm10 were plated in the PMG minimal medium without thiamine. Empty pREP1 vector was used as a control. Ctrl, a control plate-containing thiamine. b Cells overexpressing Rbm10 showed abnormal cell shapes and nuclear organization. Left, bright-field microscopic images. Right, DAPI staining. OE, overexpression. c MA plot comparing the intron retention patterns between rbm10-overexpressing cells and WT. Log2 transformed read count for each event was plotted on the X axis, and the PIR difference was on the Y axis. The red and blue dots represented the events with significantly different intron retention patterns. The grey lines indicate 10% PIR difference