Fig. 3
From: Characterization of the plant homeodomain (PHD) reader family for their histone tail interactions
dCypher histone peptide-binding assays define the PTM recognition preference of PHD proteins with high sensitivity. a–h Binding curves to determine optimal reader protein concentration for full peptide library screening on the dCypher® AlphaScreen® platform (see “Methods”). X-axes are log(protein concentration (M)) at constant peptide concentration (100 nM); Y-axes are AlphaScreen counts, representing relative strength of binding (n = 2; error bars are S.D.). i Heat map represents relative binding to H3 N-terminal peptides (left) by PHD-containing GST-tagged proteins (top) using the dCypher AlphaScreen platform. Protein concentrations can be found in Additional file 5: Table S4. Binding strength is indicated by color gradient from green to yellow (stronger to weaker). The asterisk (*) by MLL5 signifies its general preference for H3K4 methylation. Alpha counts (n = 2) were normalized individually for each protein to the highest signal for each respective assay. For full dCypher peptide screen data, see Additional file 4: Table S3