Fig. 2

A majority of PHD-containing proteins identified in the domain array are H3 K4me3 readers. The heatmap represents relative binding of the indicated H3 N-terminal peptides (left side) to the PHD-containing GST-tagged proteins (top). Binding strength is shown as a color gradient from red to blue (stronger to weaker). Most of the 31 PHD proteins preferentially recognize H3K4me3 when residues K9 and K18 are acetylated. Array signals (n = 4) were normalized individually for each protein to the highest signal for each respective array; thus, comparisons should only be made between binding strengths of different peptides for the same protein. TTP Tandem Tudor domain + PHD, PPCC Dual PHD + Dual Chromodomain, PCC PHD + Dual Chromodomain, CW CW-type Zn-finger, PB PHD + Bromodomain, PPC2W2 Dual PHD + C2W2-type Zn-finger, SPB SAND + PHD + Bromodomain; domains not indicated, one PHD finger. For full construct information, see Additional file 1: Table S1 and Additional file 2: Figure S3. For full peptide microarray data, see Additional file 3: Table S2