Fig. 4

Promoter proximal nucleosomes exhibit relatively larger differences in H2B S116C accessibility relative to the genomic average. a Genes were sorted according to AP/input scores averaged over the region TSS to 0.5 kb, then binned into quintiles, with quintile 1 containing genes with the highest AP/input scores and quintile 5 containing the lowest scores. Top, plots of cumulative AP/input scores for each quintile, as shown in the heat map (bottom). b Distributions of most and least accessible genes in replicate experiments. Shown is the distribution of genes sorted into quintiles 1 (blue) and 5 (orange) for the 1 min BM modification experiment (Fig. 3a), in quintiles of a replicate experiment