Fig. 1

a Nucleosome phasing plots for input and affinity purified (AP) samples from WT and H2B S116C cells, as indicated. Plots were aligned to the transcription start sites (TSS) of yeast genes and normalized to total reads for each data set. b Percent nucleosomes modified by biotin maleimide. Modification was carried out for 10 s, 1 and 10 min, as described in the text. Values were derived from data in Additional file 1: Table S2 as described in “Materials and methods”