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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Chromatin accessibility maps provide evidence of multilineage gene priming in hematopoietic stem cells

Fig. 3

Greater overall global accessibility of HSCs and more extensive chromatin remodeling upon lymphoid differentiation. a HSCs had the highest number of peaks of all hematopoietic cell types. The total number of individual peaks are displayed for each cell type. HSCs had the highest number of peaks followed by B cells, T cells, MkPs, EPs, then GM cells. b HSCs had the highest total accessibility signal across all peaks of all hematopoietic cell types analyzed. Average cumulative signal across the master peak-list (the number of sequencing reads that fall into the detected peaks) was determined by the -hist function of HOMER annotatePeaks.pl. c–g Comparisons of the number of peaks gained and lost upon HSC differentiation into unipotent cells revealed that MkPs had the most similar accessibility profile to HSCs. c Schematic of the pairwise comparisons made. HSC peaks were compared with one unilineage cell type at a time and those comparisons are reported in dg. d MkPs had the lowest percentage of altered peaks from HSCs compared to the other 4 unilineage cell types. The percentage of all non-overlapping peaks (peaks both gained and lost) calculated as the ratio of unique peaks in each cell type when compared pairwise to HSCs divided by the total number of peaks called in that cell type are displayed here. The numbers in the bars represent the total number of peaks altered (gained + lost) for each cell type. EPs had the highest percentage of peaks altered (gained + lost), followed by T cells, GMs, then B cells. e B cells had the highest percentage of peaks gained from HSCs, while MkPs had the lowest. Calculations as in d, but only peaks gained are shown. f EPs had the highest percentage of peaks lost from HSCs, while B cells had the least. Calculations as in d, but only peaks lost are shown. g B cells were the only lineage with more peaks gained (53%) than lost (47%) upon differentiation from HSCs. In this panel, the sum of peaks gained and lost in each cell type was set to 100% and then the ratio of peaks gained and lost was displayed. T cells had the second highest proportion of peaks gained (27%), followed by MkPs (19%), EPs (16%), then GMs (15%)

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