Fig. 2

Promoter accessibility correlated with known expression patterns of cell type-specific genes. a Lineage-specific gene expression patterns used to find all genes expressed within each unipotent lineage cell type. The level of expression (red = high; blue = low/not expressed) according to the Gene Expression Commons (GEXC) database. b Lineage-specific promoters had accessibility of the corresponding unipotent lineage cell types. Homer histograms of the average cumulative signal of all cell types used in this study across the lineage-specific promoter gene lists for EPs, MkPs, GMs, B cells, and T cells. c Lineage-specific expression of one example gene each for MkPs, EPs, B, or T cells. The level of expression (red = high; blue = low/not expressed) according to the Gene Expression Commons (GEXC) database of an example gene with cell type-specific ATAC-seq promoter peak. The probeset for the GM-specific Ly6g is not present in GEXC and therefore not displayed. d Cell type-specific chromatin accessibility visualized as ATAC-seq read-counts at transcription start sites (TSS) using UCSC Genome Browser snapshots. Depiction of the six ATAC-seq libraries used in this study with example genes that had ATAC-seq signal in all samples (GAPDH; positive control), no samples (Fezf2; negative control), or in a specific cell type: HSCs (Ndn), EPs (Klf1), MkPs (Gp6), GMs (Ly6g), B cells (CD19), and T cells (Ccr4)