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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids

Fig. 2

Characterization of antibody raised against the highly divergent C terminal domain of H1T2. a Dot-blot analysis of affinity purified antibody against H1T2 with respective peptide. b Western blot analysis of the acid extracts from rat liver (lane 1) and testes (lane 2) with antibody against H1T2. c Western blot analysis of acid-soluble proteins from 10 days postnatal (lane 1) and 40 days postnatal (lane 2) rat testes nuclei with anti-H1T2 antibodies in the presence or absence of peptide competition with a 200-fold molar excess of the peptide used for antibody generation, as indicated. Western blot analysis with anti-TP2 and anti-H3 antibodies served as positive controls. d Western blot analysis of acid extracts of epididymal sperm using H1T2 antibody. e Immunofluorescence analysis in spermatids using H1T12 antibody. f Western blot analysis of the pull-down fractions with α H1T2. Input (lane 1), anti-H1T2 antibody (lane 2), anti-H1T2 antibodies + peptide (lane 3), IgG (lane 4). No signal was observed in peptide competition and pre-immune IgG lanes

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Epigenetics & Chromatin

ISSN: 1756-8935