Fig. 1

Molecular mapping analysis of ATF7IP in mESCs. a Domain architecture of mouse ATF7IP protein. Deletion mutants used in this study are shown. b Interaction of SETDB1 with each 3xFLAG-ATF7IP expressed in Atf7ip KO mESCs. Co-IP assay was performed with the cell lines using anti-FLAG and shows that the WT and the dFNIII mutant, but not the dSETDB1 mutant, bound to SETDB1. c RT-qPCR analysis was performed with long-cultured cells after the transfection. RNA expression was normalized to Hprt expression and is shown relative to the level in WT cells. Data are mean ± SD; n = 3, technical replicates. d MSCV-GFP expression was analyzed by flow cytometric analysis at indicated time points. The transfected cells were subjected to continuous drug selection from 1 day after the transfection. e RT-qPCR analysis was performed with samples collected at day 5. RNA expression was normalized to Hprt expression and is shown relative to the level in WT cells. Data are mean ± SEM; n = 4 without IAP (n = 3) from four or three experiments. NS: P > 0.05, *P < 0.05 by unpaired Student’s t-test. f WB analysis was performed with samples collected at day 5. Comparative expression between the WT and the dFNIII mutant ATF7IP was confirmed