Fig. 1

Association of Ubp8 with chromatin depends on Spt7. a qPCR analysis of GAL1 gene expression levels in WT and spt7Δ strains. b, c Level of Ubp8-TAP (b) or Sus1-TAP (c) associated with the SAGA-regulated gene GAL1 was monitored using ChIP analysis in WT and spt7Δ strains (b, c) and in the sus1Δ strain (b only, negative control) under inhibitory (glucose) or activation conditions (galactose). d Levels of Ubp8-TAP associated with the PMA1 and YEF3 promoters were monitored using ChIP analysis in WT, sus1Δ and spt7Δ strains. In b–d promoter occupancy level was calculated as the ratio of the IP sample signal to the input signal. A.U., arbitrary units. Error bars denote the SD of at least three independent experiments in each panel. The ratios normalized with respect to an intergenic region from at least three independent experiments are shown. In a–d * indicates one-tailed unpaired Student’s t-test p-value < 0.05. e Inputs (IN) and chromatin-enriched fractions (C) of the Ubp8-TAP strain (WT) and its isogenic mutant spt7Δ, were subjected to western blotting to detect Ubp8 (α-TAP, upper panel). Enrichment of chromatin-associated proteins in the C fraction was determined by detection of total histone H2B (α-H2B, lower panel). Cropped blots are shown for clarity. Full-length blots are presented in Additional file 2: Fig. S3. All samples were run in the same gel