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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: The histone and non-histone methyllysine reader activities of the UHRF1 tandem Tudor domain are dispensable for the propagation of aberrant DNA methylation patterning in cancer cells

Fig. 5

DNA methylation maintenance is stable despite global disruptions to LIG1K126- and H3K9-associated lysine methylation signaling. a In vitro methylation of recombinant full-length LIG1 by the catalytic domain of G9a (EHMT2). 3H-SAM incorporation was monitored by autoradiography and Coomassie staining is used as a loading control. b Western blots of HCT116 cells treated with either DMSO control or the G9a inhibitor UNC0638 (1 µM) for 48 h. cbb, Coomassie brilliant blue stain of membrane. c Infinium MethylationEPIC BeadChip analysis of HCT116 cells from b. Data are presented as in Fig. 4b, c. d Western blots of HCT116 cells 12 days after incorporation of lentivirus expressing wild-type H3.3 (WT) or H3.3 K9M. e Infinium MethylationEPIC BeadChip analysis of HCT116 cells from d. Data are presented as in Fig. 4b, c

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Epigenetics & Chromatin

ISSN: 1756-8935