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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: The histone and non-histone methyllysine reader activities of the UHRF1 tandem Tudor domain are dispensable for the propagation of aberrant DNA methylation patterning in cancer cells

Fig. 3

A LIG1K126me2 cell-penetrating peptide has no significant effects on HeLa cell DNA methylation. a Fluorescence polarization binding assays between full-length UHRF1 and FAM-labeled LIG1(118–130)K126me2 peptides with (red) and without (blue) a cell-penetrating peptide (CPP) sequence, -polyethylene glycol-kkkrkv. b Fluorescence microscopy of HeLa cells after 5-h incubation with control solvent (water) or with FAM-LIG1K126me2-CPP. c Chloroalkane penetration assay (CAPA) for chloroalkane-tagged (ct) LIG1K126me2-CPP in HeLa cells (CP50), concentration where 50% of maximal penetration was observed; error bars, the standard error from the independent experiments—three independent curve fits from three independent experiments). d Infinium MethylationEPIC BeadChip analysis of HeLa cells (beta values: 0, unmethylated; 1, methylated) after 7 days of incubation with control solvent or LIG1K126me2-CPP peptide at 20 µM. Scatter plots with density for all probes (left) and those that had beta values > 0.8 in control cells (right). e Distribution of beta-value differences (∆β) between control and LIG1K126me2-CPP-treated cells for probes in panel (d) that were > 0.8 in control cells (n)

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