Fig. 9

Tandem, intergenic colocalised peaks upstream of expressed genes have characteristics of regulatory sequences. Mapped reads normalised for mapped library size from concatenated replicates of input DNA and ChIP of Pf H2A.Z, H3K18ac and H3K27ac and from matched RNAseq for schizont and ring stages. Mapped reads are plotted over the preceding gene, the upstream intergenic sequence and a gene (a) in the forward orientation and expressed in schizont stages and (b) in the reverse orientation and expressed in ring stages. c As per Fig. 7b) Average, stranded, P. falciparum TSS tag coverage at 10 and 42 h post-invasion [42] at ICPs, all other intergenic regions and gene-proximal and distal ICPs from tandem pairs of ICPs. For this comparison the proximal and distal ICPs were defined as ± 50 bp from the colocalized H2A.Z peak summit. Kruskal–Wallis test showed significant variation across all categories in both rings and schizonts (p < 0.0001), shown are P values for post hoc comparisons (Dunn’s multiple comparisons test) **** p < 0.0001, ** P < 0.001