Fig. 10

Transient transfections to detect whether intergenic colocalised peaks have gene regulatory activity. Shown are Log2 transformed nanoluciferase luminescence normalised to levels of firefly luciferase DNA and parasite genome copy number detected by qPCR. Each transfected plasmid encoded nanoluciferase with its transcription driven by upstream sequences consisting of: the putative enhancer upstream of the putative promoter (E-P); a scrambled version of the PF3D7_1362000 putative enhancer upstream of the putative promoter (Scr-P); the putative promoter alone (P); the hsp86 promoter (hsp86); an AT-matched intergenic control sequence that had no regulatory effect on neighbouring genes during the asexual lifecycle (ctrl) and a mock transfection control (no DNA). ANOVA with post hoc Sidak’s multiple comparisons test of the AT-matched control sequence to each of the putative promoter-alone constructs and the hsp86 promoter alone. *p < 0.05. Bars are the means, whiskers are the ranges