Fig. 1

Distribution of histones and their modifications throughout the intra-erythrocytic developmental cycle. a Immunofluorescence assay shows that PfH3K18ac, PfH3K27ac and PfH3K4me1 localise to the nucleus of P. falciparum in ring-, trophozoite- and schizont-stages in the IDC. Bound antibody shown in green in the first row was specific for the histone modifications indicated at the bottom of the figure. DNA was stained blue with DAPI in the second row. Bright field microscopy shows the cell morphology in the fourth row. Scale bar is 5 μm. b P. falciparum H3K4me1, H3K18ac, H3K27ac and H2A.Z levels at 0–8 hpi, 8–16 hpi, 16–24 hpi, 24–32 hpi, 32–40 hpi and 40–48 hpi during the IDC. Cell morphology at the six time points when the parasite lysates were prepared is indicated on top. H3 levels were shown as the parasite lysate loading control