Fig. 5
From: Epigenomic landscape of enhancer elements during Hydra head organizer formation
Effect of inhibition of the histone demethylase activity of KDM6A/6B on axis patterning in Hydra. a, b Effect of the pharmacological inhibitor on the ectopic tentacle phenotype which is resulted by ALP treatment. Treatment with the inhibitor of KDM6A/6B (3 µM GSK-J4) results in a significant decrease in the number of tentacles obtained due to ALP treatment. Scale bar = 500 µm. **** indicates a p value < 0.0001 estimated by a one-way ANOVA test. c Whole mount in situ hybridization for Hv_Wnt3a and HyBra1 shows decreased ectopic organizer centers (blue spots) formed when polyps are treated with GSK-J4 in combination with ALP. Scale bar = 500 µm. d Relative enrichment of H3K27ac occupancy on the neighboring genomic regions of target genes with enhanced occupancy upon ALP treatment. Upon combinatorial treatment with GSK-J4 and ALP, the level of H3K27ac shows reduced occupancy on the neighboring intergenic regions. * indicates a p value < 0.05, ** indicates a p value < 0.01 and *** indicates a p value < 0.001 using Student’s t-test (the first two bars have been reproduced from Fig. 4c to indicate changes in H3K27ac occupancy observed upon ALP treatment alone)