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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Epigenomic landscape of enhancer elements during Hydra head organizer formation

Fig. 4

Enhancer activity of cis-regulatory elements upstream to multiple Wnts and head organizer genes. Putative enhancer elements were identified based on differential modification of H3K27ac after systemic activation of Wnt signaling and validated by luciferase assay. a Plots of the genomic regions generated using IGV depicting occupancy of the H3K27ac, H3K4me3 histone modifications and RNA expression on distal genomic regions of Hv_Wnt11, Hv_Wnt5a and Hv_Pitx1. ALP-Alsterpaullone treated and VC-vehicle control. The magenta rectangles show the regions cloned into the reporter construct for performing luciferase reporter assays and quantitative ChIP-PCRs. b The relative luminescence for each of the genomic regions is plotted using pGL3-basic as the control (first column) (** indicates a p value < 0.01 using Student’s t-test). c Relative enrichment of the occupancy of H3K27ac on the neighboring intergenic regions for the target regions has been plotted as fold enrichment over the IgG control in both DMSO controls (− 5 µM ALP) and ALP treated polyps (+ 5 µM ALP) (* indicates a p value < 0.05 and ** indicates a p value < 0.01 using Student’s t-test)

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