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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Regulation of SETD2 stability is important for the fidelity of H3K36me3 deposition

Fig. 5

SETD2 forms insoluble ubiquitinated aggregates. a Microscopy images showing the colocalization of GFP-SETD2 Na fragment with RFP-Fibrillarin or RFP-Ub. The scale bar is 10 µm. b Halo purification was performed from extracts of 293T cells co-expressing Halo-C and HA-Ub. Eluted and 0.5% of Input samples were resolved on a gel and probed with the depicted antibodies. c Soluble and insoluble fractions were separated from 293T cells expressing Halo-SETD2 fragments, resolved on a gel, and probed with the depicted antibodies. *Non-specific. d Cartoon depicting the predicted low-complexity regions (LCRs) in SETD2. The prediction was performed using the webserver at https://repeat.biol.ucy.ac.cy/fgb2/gbrowse/swissprot/

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