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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: UTX/KDM6A suppresses AP-1 and a gliogenesis program during neural differentiation of human pluripotent stem cells

Fig. 3

In hNSCs, UTX promotes neuronal differentiation and suppresses glial differentiation. a IF of neuronal (MAP2 and TBR1) and glial (CD44 and GFAP) lineage markers in UTX-WT and UTX-KO cells (H7 UTX-KO and H9 UTX-KO1) at neuron time point of differentiation. b Quantification of cells that are positive for the neuronal marker TBR1 or glial/astrocytic marker GFAP. More than 250 cells were sampled for each group. * and ** indicate P < 0.05 and 0.01. c Graphs summarize RNA-seq counts per million of transcripts of neuronal and glial markers in 3 H9 UTX-WT and 3 UTX-KO (H9 UTX-KO 1–2 and H7 UTX-KO) cells undergoing neuronal differentiation. d Gene ontology analysis of downregulated genes in UTX-KO vs. UTX-WT cells undergoing neuronal differentiation. Ontology terms were ranked by P value significance, with the number of enriched genes indicated. e GSEA showed significant enrichment of axonogenesis genes in downregulated genes and astrocytic lineage genes in upregulated genes of UTX-KO vs. UTX-WT neuronal differentiation. g UTX-bound genes in hNSCs were enriched with differentially expressed genes in UTX-KO vs. UTX-WT differentiating cells. P values were calculated by the hypergeometric test, assuming normal data distribution

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