Fig. 2

UTX-KO hNSCs are more effective than UTX-WT hNSCs at forming neurospheres. a Quantification of BrdU- or PH3-positive hNSCs. For each group, 15 images of more than 17,000 cells were quantified from 1 biological experiment. Each data point represents 1 image. ns, *, and ** indicate not significant, P < 0.05, and P < 0.01, respectively, by two-sided Mann–Whitney test. b Schematic diagram of forming and serial passaging of neurospheres. c Bright-field imaging of UTX-WT hNSCs and UTX-KO cells and neurospheres. d Quantification of neurospheres through serial passaging. e GSEA showed significant enrichment of UTX-bound genes in upregulated genes in UTX-KO vs. UTX-WT neurospheres. f Gene ontology analysis of differentially expressed genes in UTX-KO neurospheres vs. cells. Ontology terms were ranked by P value significance, with the number of enriched genes indicated