Fig. 2

Characteristics of the pericentromeric Het TADs a comparative heatmap for the enrichment of various genomic and epigenomic features across scaled average Het TAD borders (30 kb) and intra-TAD regions (150 kb). TAD borders are enriched in MARs and BEAF32 while other architectural proteins are present predominantly at the intra-TAD interactions (for the p values of the overlap of features with TAD borders, see Additional file 1: Figure S5 and S6). Active histone modifications, H3K4me1, H3K4me3, H3K9ac are present within the TADs, whereas H3K36me3 and heterochromatic marks—H3K9me2/3 and HP1a—are present both at the intra-TAD and TAD border regions. b Representative snapshot showing the overlap of Het TAD organization in chr 2L with the published replication timing domains in S2 cells—late-replicating regions coincide with inactive Het TADs while active Het TADs have both early- and late-replicating regions within them