Fig. 2
DNA methylation affects chitin formation in the pupal wing discs of B. mori via BmCHT10. a Transcriptomic analysis of gene transcriptional levels in 5-aza-dC-treated wing discs of 3-day-old pupae. b Effects of 5-aza-dC treatment on the mRNA (top) and protein (bottom) levels of BmCHT10 at pupal stages. c Immunohistochemistry analyses of the effects of 5-aza-dC treatment on chitin (blue) formation and BmCHT10 protein (green) levels in the 3-day-old pupal wing disc. Scale bar: 60 μm. d Effects of 5-aza-dC treatment (left) or Dnmt1 RNAi (right) on the luciferase activity of the − 1100 to − 1 nt fragment of BmCHT10 promoter in the Bm12 cells. One microgram 5-aza-dC or dsBmDnmt1 with the vector including − 1100 to − 1 nt fragment was transfected. ddH20 or dsgfp was used as a control. Each data point is the mean ± SE of three independent assays. For the t test: p < 0.05 (*) or p < 0.01(**)