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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: N6-Adenosine methylation on mRNA is recognized by YTH2 domain protein of human malaria parasite Plasmodium falciparum

Fig. 5

Modified MeRIP analysis using PfYTH2. a The experimental flow of modified MeRIP using PfYTH2. b Pie chart represents the PfYTH2 specific MeRIP enriched reads mapped to the genome that are normalized to GST control. More than 60% of transcripts mapped to TTS region. c The motif analysis of TTS specific reads enriched in PfYTH2 samples. About 80% of reads mapped to the represented motif with a significant p-value. d Validation of the PfYTH2 specific enrichment of 6 candidate transcripts by qRT-PCR analysis. PfYTH2 and GST specific enrichment was calculated to percentage input of samples, the error bar represents SEM (n = 3). Statistical significance was calculated using paired t-test

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