Fig. 3

PfYTH2 specifically binds to m6A-containing RNA. a A representative dot blot image for the transcripts from MeRIP assay with in vitro transcribed RNA (negative control) and Pf RNA (positive control). The eluted fractions were spotted on membrane and probed with anti-m6A antibody. b The bar plot shows the intensity of spots and the distribution of data points from three independent experiments and error bar represents SEM of three biological replicate samples. The dot plot graph on the right side represents the distribution of data points from same three independent experiments. c A representative dot blot image for the transcripts from MeRIP assay with synthetic RNA oligos (positive control) with m6A modification and unmodified RNA oligos (negative control). The MeRIP assay with PfYTH2 F98A methyl-binding pocket mutant shows reduced interaction. The eluted fractions were spotted on membrane and probed with anti-m6A antibody. d The bar plot shows intensity of spots from three independent experiments and error bar represents SEM of three biological replicates. The dot plot graph on the right side represents the distribution of data points from same three independent experiments. Statistical significance was assessed using paired t-test