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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: N6-Adenosine methylation on mRNA is recognized by YTH2 domain protein of human malaria parasite Plasmodium falciparum

Fig. 1

Identification and characterization of PfYTH2 protein. a Purification of PfYTH2 protein and normalization with GST control. b A representative dot blot image for the MeRIP enriched transcripts spotted on membrane. The bar plot shows intensity of spots and error bar represents SEM of three biological replicate samples. The dot plot graph on the right side represents the distribution of data points from same three independent experiments. c Homology model for PfYTH2 is generated and methyl-binding pocket is marked with aromatic amino acids which are shown in blue color (W46, F98 and W114). The bottom image represents the surface potential analysis that shows basic amino acids patches in blue color. d Superimposed structure of PfYTH2 with m6A-containing RNA ligand shows the insertion of m6A to methyl-binding pocket. The bottom image is surface potential analysis highlighting the basic patches on PfYTH2 that forms interaction surface to negatively charged m6A-containing RNA ligand. e A representative dot blot image for MeRIP enriched transcripts with PfYTH2 wild type (WT) and mutant proteins and probed with anti-m6A antibody. f The bar plot represents the intensity of each spot from Fig. 2e and the error bar represents SEM of three biological replicate samples. The dot blot graph on the right side represents the distribution of data points from same three independent experiments and the p-values were estimated using paired t-test

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