Fig. 1

A systematic screen on the histones H2A H2B identifies residues essential for sporulation in the s228c genetic background. a Statistical analysis of the sporulation efficiency of the H2A H2B mutants. The severity of the sporulation defects is described using the following color code: a sporulation efficiency included within 0–10%, 10–25%, 25–40%, 40–80% and 80–100% is represented in purple, red, orange, grey and green, respectively. Lethal mutations are represented in black. This same color-coding is used throughout the figures of the manuscript for the s288c background. b Localization of the substitution mutants with affected sporulation efficiency. Histone fold is represented on the top of each histone based on the yeast nucleosome structure [81]