Fig. 2From: Linker histone epitopes are hidden by in situ higher-order chromatin structureImages and statistics of size distributions of H1.2 (a–c) and H1.5 (d–f) punctate chromatin domains (“chromomeres”) based on confocal (a and d) and STED (b and e) microscopy. Cells were fixed with HCHO, permeabilized with Triton X-100/PBS and blocked with 5% normal goat serum/PBS prior to immunostaining, as described earlier (24)Back to article page