Table 3 Oligonucleotides used
Name | Sequence (5′ to 3′) | Purpose (description) |
|---|---|---|
pRSdel_F | TAACTATGCGGCATCAGAGC | Cloning (Deletion of part of pRS plasmid, del4881-142) |
pRSdel_R | GGGCCTCGTGATACGCCT | |
NLS_MN_F | TTTCTTTGGCGGCATCCTGCAGCCCGGG | Cloning (Replacement of GBD sequence with NLS sequence) |
NLS_MN_R | AAGAGAAAGGTGAAAGGTCAAAGACAGTTGACTGTATCG | |
TEL05R_F (specific to TEL05R) | ACGATCGCGTCATTTTACAATG | Cloning (Amplification of XY’ from TEL05R) |
TEL05R_YP_R (non specific to TEL05R) | ACCTGATCATGCAATTAGCAAGC | |
TEL03L_F | AGCTTTCATCATTCGCGCTGA | Probing (DNA fragment specific for TEL03L) |
TEL03L_R | CGTCAACAGGTTATGAGCCCT | |
TEL06R_F | CATGAGTTCGAGTATGGTGTT | Probing (DNA fragment specific for TEL06R) |
TEL06R_R | GCATGATGATCCCCAATAAC | |
TEL05R_F | ACGATCGCGTCATTTTACAATG | Probing (DNA fragment specific for TEL05R) |
TEL05R_R | GCAGTCCTTTTGGTCAAAACC | |
Y’_XS_F | TGGAGTTTTTCAGCGTTTGCG | Probing (DNA fragment hybridizing in Y’, downstream of XhoI site, YPX probe) |
Y’_XS_R | ATCAGCATCGACAGGAATGCC | |
Y’_TR_F | TGAAAATGAAACCCTGTTCTTTAGC | Probing (DNA fragment hybridizing in Y’, encompassing Tbf1 and Reb1 binding sites, YTR probe) qPCR (Primer pair used to determine ChIP signal on Y’) |
Y’_TR_R | AACAGGGCTTGGAGGAGA | |
p05RA_oligo | TGCATTACCTTGTCATCTTCAG | Probing (Oligonucleotide used to probe p05RA plasmid) |
TRP1_F | CCGATGCTGACTTGCTGGG | Probing (DNA fragment specific for TRP1 locus) |
TRP1_R | TGCCGTAATCATTGACCAGAGCC | |
HMR-E 3f | CGAACGATCCCCGTCCAAGTTATG | qPCR (Primer pair used to determine ChIP signal on HMRE [53]) |
HMR-R 2r | TCGGAATCGAGAATCTTCGTAATGC | |
X(05R)Y_F | GGGTTGGTGGTAGGAAGTAGAGGG | qPCR (Primer pair used to determine ChIP signal on X(05R)-Y’ junction) |
X(05R)Y_R | GCAATAAGGTGACATAGATATGCTATCCTAATC | |
X(16R)Y_F | GTGTGGAATATGAAAGTAGGGTAAGTTTGAGATG | qPCR (Primer pair used to determine ChIP signal on X(16R)-Y’ junction) |
X(16R)Y_R | TCGAAGTAAAGGAGCCTACCACTC |