Table 1 Yeast strains used
Strain | Genotype | References |
|---|---|---|
W3749-1a (WT) | Mat A ade2-1 can1-100 ura3-1 his3-11,15 trp1-1 leu2-3,112 bar1∆::LEU2 | [59] |
EPY007 (MN-RAP1) | Mat alpha ade2-1 can1-100 his3-11,15 trp1-1 leu2-3,112 MN-L1-Rap1 ura3-1 (L1:linker1) | [43] |
JS311H2AMN | Mat alpha his3-200 leu2-1 met15-0 trp1-63 ura3-167 RDN1::Ty1-MET15 RDN1::mURA3-HIS3 Hta1-MN-(HA)3::kanMX6 | [45] |
EPY130 (H2A-MN) | Mat A ade2-1 can1-100 ura3-1 his3-11,15 trp1-1 leu2-3,112 bar1∆::LEU2 Hta1-MN-(HA)3::kanMX6 | This study |
MVL054 | EPY007 + sir4Δ::kanMX4 | [43] |
EPY070 | EPY007 + yku80Δ::LEU2 | This study |
EPY031 | W3749-1a + sir4Δ::kanMX4 | This study |
EPY129 | W3749-1a + yku80∆::URA3 | This study |
EPY131 | EPY007 + sir3Δ::HIS3 | This study |
MVY221 (yKU70-MN) | Mat A Yku70-MN::TRP1 ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 | [43] |
MVL047 | MVY221 + bar1Δ::natMX4 + sir4Δ::kanMX4 | [43] |