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Table 1 Capabilities of BAMScale and other publicly available tools

From: BAMscale: quantification of next-generation sequencing peaks and generation of scaled coverage tracks

 

Tool

 

BAMscale

IGVtools

bedtools

MACS (callpeak followed by bedgraph2bigwig (UCSC)**

MACS (pileup followed by bedgraph2bigwig (UCSC)**

deeptools

Creating coverage tracks

 ChIP-seq/ATAC-seq

X

X

 

X

*

X

 ChIP-seq/ATAC-seq (normalized)

X

  

X

 

X

 Log2 coverage (replication timing)

X

    

X

 OK-seq (RFD calculation)

X

     

 RNA-seq

X

*

 

*

*

X

 RNA-seq (splice-aware)

X

     

 Stranded coverage

X

    

X

Quantifying peaks

 Raw read counts

x

 

x

   

 Normalized read counts

x

     
  1. * Scaling factor cannot be specified
  2. ** BAM file has to be pre-filtered for alignment quality