Fig. 3
From: DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices
Nanochannel stretching of YOYO-1 stained DNA under MBD2FLsc binding. a Fluorescence images of methylated DNA (left), methylated DNA with labeled MBD2FLsc (center, ATTO 565-labeled MBD2FLsc in red and methylated \(\lambda\)-DNA in green), and methylated DNA with unlabeled MBD2FLsc (right). b Fluorescence images of unmethylated DNA (left), unmethylated DNA with labeled MBD2FLsc (center with ATTO 565 labeled MBD2FLsc in red and methylated \(\lambda\)-DNA in green), and unmethylated DNA with unlabeled MBD2FLsc (right). The scale bar represents \({5}\,\upmu \hbox {m}\). c Histograms of \(R_g\) for methylated DNA (blue: bare DNA, N=44, magenta: ATTO 565-MBD2FLsc, N=35, yellow: MBDFLsc, N=38). d Histograms of \(R_g\) for unmethylated DNA (blue: bare DNA, N=27, magenta: ATTO 565-MBD2FLsc, N=34, yellow: MBDFLsc, N=30)