Fig. 2From: DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devicesFluorescence anisotropy titration of protein binding to \({24}\,\hbox {nM}\) methylated dsDNA substrate. a Labeled (blue *) and unlabeled MBD2FLsc (red •) yielded affinities of \(24.5\,\pm 4.6\,\hbox {nM}\) and \(11.9\,\pm 3.8\,\hbox {nM}\), respectively. b MeCP2 yielded an affinity of \(7.5\,\pm \,4.3\,\hbox {nM}\). Error bars are standard deviations over 3 measurementsBack to article page