Fig. 4

A subset of regions with increased chromatin accessibility in Setd8 mutant cells are marked by the repressive heterochromatin mark H3K27me3 in wild-type erythroblasts. a Heat map of normalized coverage depicting H3K27me3 occupancy at regions of more accessible chromatin in Setd8 mutant erythroblasts. b Chromatin accessibility and H3K27me3 occupancy at the Hlx and Hhex loci. c Genomic distribution of regions more accessible in Setd8 mutant that contain significant enrichments of H3K27me3 in normal erythroblasts. Results are compared to the composition of the whole genome at baseline. d Overlap of genes expressed at higher levels in multipotent progenitors compared to proerythroblasts with genes expressed more highly in Setd8 mutant cells compared to control. e scRNA-Seq performed on Kit+ hematopoietic progenitors from the mouse bone marrow confirms that candidate genes (Hhex and Hlx) are members of the multilineage transcriptome that are repressed during erythroid specification. Brighter green color indicates increased RNA expression. Data were adapted from: https://kleintools.hms.harvard.edu/paper_websites/tusi_et_al/; [2])