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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Linker histone variant H1t is closely associated with repressed repeat-element chromatin domains in pachytene spermatocytes

Fig. 2

Localization of linker histone variant H1t at TSS, active gene promoters, recombination hotspots and open chromatin regions (ATAC-seq-positive regions). a Workflow of the experimental protocol used for the H1t ChIP-sequencing technique. b Chromosome-wide distribution of H1t peaks in the pachytene genome. The y-axis represents the fold enrichment observed for H1t IP over background input, while the x-axis represent the various H1t-occupied locations along the length of each chromosome. The normalization of high fold enriched peaks has been done to 3.5-fold change and the lower threshold has been maintained for fold change = 2. Analysis of overlap between H1t and c TSS of the mouse (GENCODE), d H3K4me3 common representing the TSS-associated H3K4me3 marks (GSE93955), e DSB hotspots (GSE93955), f open chromatin regions of pachytene spermatocytes [35]. H1t were observed to be not enriched at active gene promoters, TSS regions, and open chromatin genomic regions. Information in cf represent the overlap that has been determined using aggregation plots (left panels) and heat maps (right panels)

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