Fig. 7
From: In vivo visualization of the i-motif DNA secondary structure in the Bombyx mori testis
Effects of different pH values on i-motif formation in the B. mori testis. Testes were isolated from 5th instar larvae and treated as described in “Materials and Methods” section. a Analysis of the intracellular pHrodo Red fluorescence intensity in testis cells cultured in media with different pH values. A higher fluorescence intensity indicates a lower pH value. b–e The pH values of the testis cell culture medium were adjusted with HCl and NaOH to 5.00, 6.02, 7.13 and 8.00 before immunofluorescence detection. The red fluorescence signals show the binding of BmILF to i-motif structure. Nuclei were counterstained with DAPI (blue). f Quantification of i-motif staining signals for 50 nuclei in five randomly selected observation regions on the slide. The data are represented as the mean ± SEM of three replicates. g qRT-PCR analysis of the pH effects on the expression of BmPOUM2. h qRT-PCR analysis of the pH effects on the expression of BGIBMGA003213