Fig. 3
From: In vivo visualization of the i-motif DNA secondary structure in the Bombyx mori testis
The binding affinity of BmILF to i-motif structures. EMSA for the binding of the recombinant BmILF to the i-motif probes of BmPOUM2 (A) and BGIBMGA003213 (B) at different concentrations of the probe (Aa, Ba) or protein (Ab, Bb). Quantitative measurement of the binding band of BmILF and i-motif of BmPOUM2 (Ac) and BGIBMGA003213 (Bc). Hill curves and the Kd values of the binding of BmILF and i-motif probes of BmPOUM2 (Ad) and BGIBMGA003213 (Bd). MST analysis for the binding affinity of the recombinant BmILF with the i-motif of BmPOUM2 (C) and BGIBMGA003213 (D) at different protein concentrations