Fig. 6

Depletion of BRD4 or BRD2 in Melb-a cells suppresses expression of melanocyte-specific genes. a Melb-a cells were transfected with control siRNA or siRNA that targets BRD2, BRD3, or BRD4, then differentiated for 48 h. Immunoblot analysis was performed with the indicated antibodies. The immunoblot is representative of two or more experiments. b Melb-a cells were transfected with a control (siC) or a pool of siRNAs that targets BRD4 or BRD2 (distinct from those used in a) then differentiated for 48 h. Immunoblot analysis was performed with the indicated antibodies. The immunoblot is representative of two or more experiments. c, d BRD4 or BRD2 depleted cells treated as described in b were subjected to qRT-PCR. Brd4, Brd2, Tyr, and Tyrp1 levels were normalized to that of Rpl7. The data in c and d are the average of three independent experiments. Standard error bars are shown. Statistically significant differences between siC, siBRD4, and siBRD2 transfected cells are shown (*p < 0.05, **p < 0.01, ***p < 0.001)