Fig. 5

Melanocyte differentiation gene expression is suppressed by BET inhibition. a Melb-a cells were differentiated in the presence of vehicle (VC) or 500 nM (+)JQ1. Cells were harvested at the indicated time-points and subjected to qRT-PCR. Tyr, Tyrp1, or Trpm1 levels were normalized to that of Rpl7. The data are the average of three independent experiments. Standard error bars are shown. Statistically significant difference between VC and (+)JQ1 treated samples at each time point are shown (*p < 0.05, **p < 0.01, ***p < 0.001). b Protein extracts from cells cultured in the absence (vehicle treated) or presence of (+)JQ1 were subjected to immunoblot analysis using the indicated antibodies. The figure is representative of three or more experiments. c Neonatal human epidermal melanocytes (NHEMs) were cultured in the absence (vehicle treated) or presence of 500 nM (+)JQ1 for the indicated number of days. Harvested cells were subjected to immunoblot analysis using the indicated antibodies. The figure is representative of three or more experiments. d NHEMs were cultured as in c and then subjected to qRT-PCR. The TYRP1 level was normalized to that of RPL9. The data are the average of three independent experiments. Standard error bars are shown. Statistically significant difference between VC and (+)JQ1 treated samples at each time point are shown (*p < 0.05, **p < 0.01, ***p < 0.001)