Fig. 5

DNE elicits multigenerational HDAC2 deficits in the frontal cortices and hippocampi and alters HDAC2 (Ser³⁹⁴) phosphorylation in the frontal cortices, striata, and hippocampi. Representative Western blot images and densitometric measurements of HDAC2 abundance and fractional HDAC2 (Ser³⁹⁴) phosphorylation in frontal cortices, striata, and hippocampi. a HDAC2 abundance in frontal cortices (n_F1Veh = 14, n_F1NIC = 11, and n_F2NIC = 11). F1 NIC and F2 NIC mice have reduced frontal cortical HDAC2 content. b HDAC2 abundance in striata (n_F1Veh = 13, n_F1NIC = 12, and n_F2NIC = 12). Striatal HDAC2 content is unaltered in F1 NIC and F2 NIC mice. c HDAC2 abundance in hippocampi (n_F1Veh = 12, n_F1NIC = 13, and n_F2NIC = 13). F1 NIC and F2 NIC mice have reduced hippocampal HDAC2 content (d) Fractional HDAC2 (Ser³⁹⁴) phosphorylation in frontal cortices (n_F1Veh = 6, n_F1NIC = 5, and n_F2NIC = 5). F1 NIC and F2 NIC mice have increased frontal cortical fractional HDAC2 (Ser³⁹⁴) phosphorylation. e Fractional HDAC2 (Ser³⁹⁴) phosphorylation in striata (n_F1Veh = 5, n_F1NIC = 6, and n_F2NIC = 5). F1 NIC and F2 NIC mice have reduced striatal fractional HDAC2 (Ser³⁹⁴) phosphorylation. f Fractional HDAC2 (Ser³⁹⁴) phosphorylation in hippocampi (n_F1Veh = 6, n_F1NIC = 5, and n_F2NIC = 5). F1 NIC and F2 NIC mice have increased hippocampal fractional HDAC2 (Ser³⁹⁴) phosphorylation. FCX frontal cortices, STR striata, HIPP hippocampi, HDAC2 histone deacetylase 2, total total HDAC2, Phospho phospho-HDAC2 (Ser³⁹⁴), TBP TATA-binding protein. All data are mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001