Fig. 4

DNE elicits multigenerational MeCP2 deficits in the frontal cortices and hippocampi. Representative Western blot images and densitometric measurements of MeCP2 abundance and fractional MeCP2 (Ser⁴²¹) phosphorylation in frontal cortices, striata, and hippocampi. a MeCP2 abundance in frontal cortices (n_F1Veh = 11, n_F1NIC = 10, and n_F2NIC = 11). F1 NIC and F2 NIC mice have reduced frontal cortical MeCP2 content. b MeCP2 abundance in striata (n_F1Veh = 12, n_F1NIC = 10, and n_F2NIC = 10). Striatal MeCP2 content is unaltered in F1 NIC and F2 NIC mice. c MeCP2 abundance in hippocampi (n_F1Veh = 13, n_F1NIC = 14, and n_F2NIC = 13). F1 NIC and F2 mice NIC have reduced hippocampal MeCP2 content. d Fractional MeCP2 (Ser⁴²¹) phosphorylation in frontal cortices (n_F1Veh = 7, n_F1NIC = 6, and n_F2NIC = 7). Frontal cortical fractional MeCP2 (Ser⁴²¹) phosphorylation is unaltered in F1 NIC and F2 NIC mice. e Fractional MeCP2 (Ser⁴²¹) phosphorylation in striata (n_F1Veh = 8, n_F1NIC = 8, and n_F2NIC = 8). Striatal fractional MeCP2 (Ser⁴²¹) phosphorylation is unaltered in F1 NIC and F2 NIC mice. f Fractional MeCP2 (Ser⁴²¹) phosphorylation in hippocampi (n_F1Veh = 8, n_F1NIC = 6, and n_F2NIC = 9). Hippocampal fractional MeCP2 (Ser⁴²¹) phosphorylation is unaltered in F1 NIC and F2 NIC mice. FCX frontal cortices, STR striata, HIPP hippocampi, MeCP2 methyl-CpG binding protein 2, total total MeCP2, phospho phospho-MeCP2 (Ser⁴²¹), TBP TATA-binding protein. All data are mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001