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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability

Fig. 2

tNASP overexpression mimics the H3 accumulation observed upon siJMJD1B. a Western blot of 5 and 15 µg of cytosolic extracts derived from siControl, siNASP, and/or siJMJD1B treated HeLa cells. b Western blot of 5 and 10 µg of cytosolic extracts derived from Mock or overexpressed-Flag-tNASP HeLa cells. The graph shows the fold change of proteins in the Flag-tNASP conditions, respect to Mock, normalized by β-actin. The standard deviation was obtained from three independent experiments. **p < 0.01, ***p < 0.001, Student’s t-test. c Western blot of 20 µg of total cell extracts derived from siControl or siJMJD1B HeLa cells that were treated for 0 to 7 h with 100 µg/mL of cycloheximide (CHX). The graphs show the mean value of each protein, normalized by β-actin at each time-point, from three independent experiments. d Western blot of 20 µg of total cell extracts derived from HeLa cells treated with vehicle (DMSO), 100 µg/mL CHX, or 100 µg/mL CHX plus protein-degradation inhibitors: 50 µM MG132 (ubiquitin–proteasome pathway) or 20 mM NH4Cl + 200 µM leupeptin (lysosomal pathways)

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