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Table 2 PCR primer sequences for bisulfite sequencing analysis

From: Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus

 NameSequences
5′ primerICR-MA-5S45′-GAATTTGGGGTATTTAAAGTTTTG-3′
ICR-MA-5S135′-GGTGATTTATAGTATTGTTATTTG-3′
LCR-MA-5S15′-TATAGATGTTTTAGTTTTAATAAG-3′
lambda-MA-5S15′-ATTAGTAAGAAGATAGTAGTGATG-3′
lambda-MA-5S45′-TTAAGTTTTGTGTGTTATTTATTA-3′
lambda-MA-5S55′-GTTAAAAAGAAGAAGTAAGTATTT-3′
lambda-MA-5S65′-GTGAAAGTATTGATTATTATGTTA-3′
lambda-MA-5S75′-GAGGTTTATTTGTATTTATTTTTGTT-3′
lambda-MA-5S85′-TATTTTTTAGTAGTATTGTAAGAGGT-3′
3′ primerICR-MA-3A25′-AACAATACTAAATCTACCTAAAAC-3′
ICR-MA-3A145′-AAAACATAAAAACTATTATATACA-3′
ICR-MA-3A155′-ACCAACCAATATAACTCACTATAA-3′
ICR-MA-3A215′-ACTTACTACCCTCATTATACTTT-3′
ICR-MA-3A265′-CAAATTAACAAAAACATACCTAACT-3′
BGLB-MA-3A25′-TTCTAACCCCACAAAAATTTATTC-3′
BGLB-MA-3A65′-CCAAACCCCCTCTATTTTATATCA-3′
lambda-MA-3A15′-CTAACATTTATCTACATCATACCT-3′
lambda-MA-3A25′-ATACCTTATTTTTTTCTACTACAA-3′
lambda-MA-3A35′-CTAAACTCCAACATATAATAACCC-3′
lambda-MA-3A75′-AACCAAAATTATCTTTTTCTATCT-3′
lambda-MA-3A85′-ACAACATTCTTAAATCCAATATTA-3′