Table 2 PCR primer sequences for bisulfite sequencing analysis
| Â | Name | Sequences |
|---|---|---|
5′ primer | ICR-MA-5S4 | 5′-GAATTTGGGGTATTTAAAGTTTTG-3′ |
ICR-MA-5S13 | 5′-GGTGATTTATAGTATTGTTATTTG-3′ | |
LCR-MA-5S1 | 5′-TATAGATGTTTTAGTTTTAATAAG-3′ | |
lambda-MA-5S1 | 5′-ATTAGTAAGAAGATAGTAGTGATG-3′ | |
lambda-MA-5S4 | 5′-TTAAGTTTTGTGTGTTATTTATTA-3′ | |
lambda-MA-5S5 | 5′-GTTAAAAAGAAGAAGTAAGTATTT-3′ | |
lambda-MA-5S6 | 5′-GTGAAAGTATTGATTATTATGTTA-3′ | |
lambda-MA-5S7 | 5′-GAGGTTTATTTGTATTTATTTTTGTT-3′ | |
lambda-MA-5S8 | 5′-TATTTTTTAGTAGTATTGTAAGAGGT-3′ | |
3′ primer | ICR-MA-3A2 | 5′-AACAATACTAAATCTACCTAAAAC-3′ |
ICR-MA-3A14 | 5′-AAAACATAAAAACTATTATATACA-3′ | |
ICR-MA-3A15 | 5′-ACCAACCAATATAACTCACTATAA-3′ | |
ICR-MA-3A21 | 5′-ACTTACTACCCTCATTATACTTT-3′ | |
ICR-MA-3A26 | 5′-CAAATTAACAAAAACATACCTAACT-3′ | |
BGLB-MA-3A2 | 5′-TTCTAACCCCACAAAAATTTATTC-3′ | |
BGLB-MA-3A6 | 5′-CCAAACCCCCTCTATTTTATATCA-3′ | |
lambda-MA-3A1 | 5′-CTAACATTTATCTACATCATACCT-3′ | |
lambda-MA-3A2 | 5′-ATACCTTATTTTTTTCTACTACAA-3′ | |
lambda-MA-3A3 | 5′-CTAAACTCCAACATATAATAACCC-3′ | |
lambda-MA-3A7 | 5′-AACCAAAATTATCTTTTTCTATCT-3′ | |
lambda-MA-3A8 | 5′-ACAACATTCTTAAATCCAATATTA-3′ |