Table 1 Primer sets for bisulfite sequencing analysis
Regions analyzed | Allele | PCR round | 5′ primer | 3′ primer | |
|---|---|---|---|---|---|
I | del-6 to del-8 Tg-5′ICR-KO(116) | Nested PCR | 1st | LCR-MA-5S1 | ICR-MA-3A15 |
2nd | ICR-MA-5S4 | ICR-MA-3A14 | |||
II | del-6 to del-8 | Nested PCR | 1st | ICR-MA-5S13 | BGLB-MA-3A2 |
2nd | ICR-MA-5S13 | ICR-MA-3A2 | |||
III | Tg-5′ICR-KO(116) | Nested PCR | 1st | ICR-MA-5S13 | BGLB-MA-3A2 |
2nd | ICR-MA-5S13 | ICR-MA-3A26 | |||
III′ | endo-5′ICR-KO(116) | Single-round PCR |  | ICR-MA-5S13 | ICR-MA-3A26 |
IV | Tg-LCb Tg-LCb118 | Nested PCR | 1st | lambda-MA-5S4 | lambda-MA-3A2 |
2nd | lambda-MA-5S1 | lambda-MA-3A3 | |||
V | Tg-LCb Tg-LCb118 | Nested PCR | 1st | lambda-MA-5S5 | lambda-MA-3A7 |
2nd | lambda-MA-5S6 | lambda-MA-3A8 | |||
VI | Tg-LCb | Nested PCR | 1st | lambda-MA-5S7 | BGLB-MA-3A6 |
2nd | lambda-MA-5S8 | BGLB-MA-3A2 | |||
VII | Tg-LCb118 | Nested PCR | 1st | lambda-MA-5S7 | BGLB-MA-3A6 |
2nd | lambda-MA-5S8 | BGLB-MA-3A2 | |||
VIII | Endo-LCb Endo-LCb118 | Nested PCR | 1st | lambda-MA-5S7 | ICR-MA-3A21 |
2nd | lambda-MA-5S8 | lambda-MA-3A1 | |||