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Table 1 Primer sets for bisulfite sequencing analysis

From: Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus

Regions analyzedAllelePCR round5′ primer3′ primer
Idel-6 to del-8
Tg-5′ICR-KO(116)
Nested PCR1stLCR-MA-5S1ICR-MA-3A15
2ndICR-MA-5S4ICR-MA-3A14
IIdel-6 to del-8Nested PCR1stICR-MA-5S13BGLB-MA-3A2
2ndICR-MA-5S13ICR-MA-3A2
IIITg-5′ICR-KO(116)Nested PCR1stICR-MA-5S13BGLB-MA-3A2
2ndICR-MA-5S13ICR-MA-3A26
III′endo-5′ICR-KO(116)Single-round PCR ICR-MA-5S13ICR-MA-3A26
IVTg-LCb
Tg-LCb118
Nested PCR1stlambda-MA-5S4lambda-MA-3A2
2ndlambda-MA-5S1lambda-MA-3A3
VTg-LCb
Tg-LCb118
Nested PCR1stlambda-MA-5S5lambda-MA-3A7
2ndlambda-MA-5S6lambda-MA-3A8
VITg-LCbNested PCR1stlambda-MA-5S7BGLB-MA-3A6
2ndlambda-MA-5S8BGLB-MA-3A2
VIITg-LCb118Nested PCR1stlambda-MA-5S7BGLB-MA-3A6
2ndlambda-MA-5S8BGLB-MA-3A2
VIIIEndo-LCb
Endo-LCb118
Nested PCR1stlambda-MA-5S7ICR-MA-3A21
2ndlambda-MA-5S8lambda-MA-3A1