Fig. 7

Reconstitution of the differential methylation in the LCb118 sequence at the mouse endogenous Igf2/H19 locus. a Map of the wild-type and knock-in alleles. The endogenous mouse H19 ICR sequence was replaced by the LCb or LCb118 synthetic DNA fragments. Filled and gray boxes indicate the “b” region (which includes Sox-Oct motifs) and 118-bp sequence, respectively. DNA methylation status of the LCb (b) and LCb118 (c) sequence in sperm, 2-cell embryos, and blastocysts of knock-in mice, that inherited the mutant alleles either paternally (pat.) or maternally (mat.), was analyzed by bisulfite sequencing. The overall percentage of methylated CpGs is indicated next to each panel