Fig. 4

The 118-bp sequence was also required for paternal methylation of the endogenous H19 ICR. a Map of wild-type and knockout alleles. The same 116-bp sequence that was deleted from the transgenic H19 ICR fragment in Fig. 3, was removed from the mouse endogenous locus by CRISPR/Cas9 genome editing (endo-5′ICR-KO(116)). b–d DNA methylation status of the mutant H19 ICR in sperm (b), 2-cell embryos (c), or blastocysts (d) of 116-bp KO mice, that inherited the KO allele either paternally (pat.) or maternally (mat.), was analyzed by bisulfite sequencing