Fig. 3

The 118-bp sequence was required for paternal methylation of the transgenic H19 ICR. a Structure of the transgenes. The 116-bp sequence, which was a part of the 118-bp region identified by 5′-truncation experiments (Fig. 2), was internally deleted from the 2.9-kb H19 ICR fragment in TgM by CRISPR/Cas9 genome editing (Tg-5′ICR-KO(116)). Regions I and III, indicated by gray bars below the map, were analyzed by bisulfite sequencing. b DNA methylation status of the mutant H19 ICR transgene in tail somatic cells (upper panel) or 2-cell embryos (lower) of TgM, that inherited the transgenes either paternally (pat.) or maternally (mat.), was analyzed by bisulfite sequencing