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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus

Fig. 1

Search for DNA sequences which are responsible for paternal methylation of the H19 ICR fragment. a Structure of the mouse endogenous Igf2/H19 locus. The expression of paternal Igf2 and maternal H19 genes depends on the shared 3′ enhancer. The H19 ICR, located approximately at − 4 to − 2 kb relative to the transcription start site of H19 gene is contained within a 2.9-kb SacI (Sa)-BamHI (B) fragment. DNA sequence (478 bp) shown to be sufficient for acquiring paternal methylation in TgM [15] is marked in gray. Dots (1–4) indicate CTCF-binding sites. G, BglII; H, HindIII sites. b Structure of the 150-kb human β-globin locus YAC. The LCR and β-like globin genes are denoted as gray and filled boxes, respectively. In our previous studies, the H19 ICR (2.9-kb) or the ICR4321S (766-bp shorter than the original 2.9-kb sequence) fragments were introduced 3′ to the LCR [11, 12]. In this study, a series of 5′-truncated H19 ICR fragments (del-2–9) were inserted into the identical position of the YAC to examine their activities in TgM

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