Fig. 1
From: Nickel-induced transcriptional changes persist post exposure through epigenetic reprogramming
Nickel exposure causes persistent gene expression changes. a Schematic representation of nickel exposure. BEAS-2B cells were treated with 100 μM NiCl2 for 6 weeks (nickel-exposed). After exposure, the cells were plated at colony forming density to obtain populations of cells originating from individual nickel-exposed cells and grown in nickel-free medium for > 6 months (nickel-washed-out). Gene expression was examined using RNA-Seq. b RNA-Seq results displayed as a heatmap showing the persistently and transiently differentially expressed genes (1.5-fold up- or down-regulation; Padj < 0.05) in untreated (UT), nickel-exposed (Ni-E) and Ni-washed-out (Ni-W) cells. TU transiently upregulated genes, PU-A genes persistently upregulated in the presence of nickel, PU-B genes persistently upregulated after the termination of exposure, TD transiently downregulated genes, PD-A genes persistently downregulated in the presence of nickel, PD-B genes persistently downregulated after the termination of exposure. Normalized log2-transformed RPKM (pseudo-count = 1) values were used to generate the heatmap